Journal: Synthetic and Systems Biotechnology

Article Title: Multi-omics analyses of evolved Corynebacterium glutamicum mutants reveal the molecular responses to formaldehyde stress

doi: 10.1016/j.synbio.2026.01.020

Figure Lengend Snippet: Improving the tolerance to formaldehyde via ALE. (A) Growth of FM-1 in minimal medium with 10 g/L glucose and different formaldehyde concentrations. 0 mM (square), 0.5 mM (triangle), 0.8 mM (circle), and 1 mM (inverted triangle). (B) ALE procedure of culture-1 in CGXII minimal medium supplemented with different formaldehyde concentrations and 10 g/L glucose. (C) Growth curve of the evolved mutants in CGXII minimal medium supplemented with 10 g/L glucose and 0.8 mM formaldehyde. (D) Growth curve of evolved mutant in CGXII minimal medium supplemented with 10 g/L glucose and 1.6 mM formaldehyde. (E) Growth curve of evolved mutant in CGXII minimal medium supplemented with 10 g/L glucose. (F) Formaldehyde degradation during cell growth of wild-type C. glutamicum ATCC 13032, FM-1 and FM-3. Values and error bars reflect the mean ± s.d. of three biological replicates (N = 3).

Article Snippet: Each evolved strain possesses more than 100 mutations including single nucleotide polymorphisms (SNPs), insertions, and deletions, which were aligned against the C. glutamicum ATCC 13032 reference genome (GenBank accession number GCA_000011325.1).

Techniques: Mutagenesis

Journal: Journal of Biomedical Science

Article Title: Phage–antibiotic synergy restores β-lactam efficacy in MDR Klebsiella quasipneumoniae biofilms and suppresses resistance

doi: 10.1186/s12929-026-01218-1

Figure Lengend Snippet: Assessment of PAS effects on planktonic K. quasipneumoniae ATCC 700603. A – E Bacterial growth curves under different treatment conditions (as indicated in the diagrams). The concentrations of CAZ as the fold change in the MIC (MIC CAZ = 32 mg/L) and phage titers are indicated in the legends. The data are presented as the means ± standard deviations (SDs, n = 3)

Article Snippet: Furthermore, this study utilizes a single-strain–single-phage model centered on the reference strain ATCC 700603.

Techniques:

Journal: Journal of Biomedical Science

Article Title: Phage–antibiotic synergy restores β-lactam efficacy in MDR Klebsiella quasipneumoniae biofilms and suppresses resistance

doi: 10.1186/s12929-026-01218-1

Figure Lengend Snippet: Quantitative evaluation of treatment effects on K. quasipneumoniae ATCC 700603 biofilms following exposure to CAZ (0.25 × MIC), phage vB_KpUKJ_2 (10 8 PFU/mL), or their combination. A Surface area covered (SAC) by viable biofilm (in %) over time, as determined by the calcein fluorescence signal after staining with CAM. A total pixel area of 2048 × 2048 µm was analyzed from LSFM micrographs; B AUC representation of the kinetic data from ( A ) over a treatment period of 0–24 h, based on relative fluorescence units (RFU) × h; C Viable bacterial counts (CFU/mL) after biofilm treatments at three distinct time points. Statistical analysis was performed via two-way ANOVA followed by Tukey’s multiple comparisons test. D Phage particles (PFU/mL) after combined biofilm treatment at three time points. Statistical analysis was conducted via one-way ANOVA followed by Dunnett’s post hoc test. The data are presented as the means ± SDs (n = 3). Significance was assumed at p < 0.05 and is indicated by asterisks: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001

Article Snippet: Furthermore, this study utilizes a single-strain–single-phage model centered on the reference strain ATCC 700603.

Techniques: Fluorescence, Staining

Journal: Journal of Biomedical Science

Article Title: Phage–antibiotic synergy restores β-lactam efficacy in MDR Klebsiella quasipneumoniae biofilms and suppresses resistance

doi: 10.1186/s12929-026-01218-1

Figure Lengend Snippet: Representative CLSM images showing the effects of phage vB_KpUKJ_2 (10 8 PFU/mL) on mature K. quasipneumoniae ATCC 700603 biofilms at 0, 4, and 24 h post-treatment. Biofilms were stained with Con-A (green) for α-polysaccharides or with CFW (magenta) for β-polysaccharides to visualize extracellular matrix integrity. A Untreated control, B phage-treated samples. To minimize photobleaching and laser-induced biofilm disruption, independent samples were used for each time point. Each image represents a representative Z-stack projection from three independent experiments. Scale bars: 20 μm

Article Snippet: Furthermore, this study utilizes a single-strain–single-phage model centered on the reference strain ATCC 700603.

Techniques: Staining, Control, Disruption

Journal: Journal of Biomedical Science

Article Title: Phage–antibiotic synergy restores β-lactam efficacy in MDR Klebsiella quasipneumoniae biofilms and suppresses resistance

doi: 10.1186/s12929-026-01218-1

Figure Lengend Snippet: Characteristics of six phage-resistant mutants in comparison with the parental K. quasipneumoniae ATCC 700603 strain. A Differences in the AUCs based on growth kinetics. B Biofilm formation ability at 48 h quantified by crystal violet staining and absorbance measured at 570 nm; the data are presented as the means ± SDs (n = 3). Statistical analyses were performed via one-way ANOVA followed by Tukey’s multiple comparisons test. Significance was assumed at p < 0.05 and is indicated by asterisks: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0. 0001

Article Snippet: Furthermore, this study utilizes a single-strain–single-phage model centered on the reference strain ATCC 700603.

Techniques: Comparison, Staining

Journal: Journal of Biomedical Science

Article Title: Phage–antibiotic synergy restores β-lactam efficacy in MDR Klebsiella quasipneumoniae biofilms and suppresses resistance

doi: 10.1186/s12929-026-01218-1

Figure Lengend Snippet: Assessment of PAS effects on planktonic K. quasipneumoniae ATCC 700603. A – E Bacterial growth curves under different treatment conditions (as indicated in the diagrams). The concentrations of CAZ as the fold change in the MIC (MIC CAZ = 32 mg/L) and phage titers are indicated in the legends. The data are presented as the means ± standard deviations (SDs, n = 3)

Article Snippet: To identify genetic determinants of phage resistance, we performed whole-genome sequencing on resistant isolates and mapped the reads to the parental K. quasipneumoniae ATCC 700603 reference genome.

Techniques:

Journal: Journal of Biomedical Science

Article Title: Phage–antibiotic synergy restores β-lactam efficacy in MDR Klebsiella quasipneumoniae biofilms and suppresses resistance

doi: 10.1186/s12929-026-01218-1

Figure Lengend Snippet: Quantitative evaluation of treatment effects on K. quasipneumoniae ATCC 700603 biofilms following exposure to CAZ (0.25 × MIC), phage vB_KpUKJ_2 (10 8 PFU/mL), or their combination. A Surface area covered (SAC) by viable biofilm (in %) over time, as determined by the calcein fluorescence signal after staining with CAM. A total pixel area of 2048 × 2048 µm was analyzed from LSFM micrographs; B AUC representation of the kinetic data from ( A ) over a treatment period of 0–24 h, based on relative fluorescence units (RFU) × h; C Viable bacterial counts (CFU/mL) after biofilm treatments at three distinct time points. Statistical analysis was performed via two-way ANOVA followed by Tukey’s multiple comparisons test. D Phage particles (PFU/mL) after combined biofilm treatment at three time points. Statistical analysis was conducted via one-way ANOVA followed by Dunnett’s post hoc test. The data are presented as the means ± SDs (n = 3). Significance was assumed at p < 0.05 and is indicated by asterisks: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001

Article Snippet: To identify genetic determinants of phage resistance, we performed whole-genome sequencing on resistant isolates and mapped the reads to the parental K. quasipneumoniae ATCC 700603 reference genome.

Techniques: Fluorescence, Staining

Journal: Journal of Biomedical Science

Article Title: Phage–antibiotic synergy restores β-lactam efficacy in MDR Klebsiella quasipneumoniae biofilms and suppresses resistance

doi: 10.1186/s12929-026-01218-1

Figure Lengend Snippet: Representative CLSM images showing the effects of phage vB_KpUKJ_2 (10 8 PFU/mL) on mature K. quasipneumoniae ATCC 700603 biofilms at 0, 4, and 24 h post-treatment. Biofilms were stained with Con-A (green) for α-polysaccharides or with CFW (magenta) for β-polysaccharides to visualize extracellular matrix integrity. A Untreated control, B phage-treated samples. To minimize photobleaching and laser-induced biofilm disruption, independent samples were used for each time point. Each image represents a representative Z-stack projection from three independent experiments. Scale bars: 20 μm

Article Snippet: To identify genetic determinants of phage resistance, we performed whole-genome sequencing on resistant isolates and mapped the reads to the parental K. quasipneumoniae ATCC 700603 reference genome.

Techniques: Staining, Control, Disruption

Journal: Journal of Biomedical Science

Article Title: Phage–antibiotic synergy restores β-lactam efficacy in MDR Klebsiella quasipneumoniae biofilms and suppresses resistance

doi: 10.1186/s12929-026-01218-1

Figure Lengend Snippet: Characteristics of six phage-resistant mutants in comparison with the parental K. quasipneumoniae ATCC 700603 strain. A Differences in the AUCs based on growth kinetics. B Biofilm formation ability at 48 h quantified by crystal violet staining and absorbance measured at 570 nm; the data are presented as the means ± SDs (n = 3). Statistical analyses were performed via one-way ANOVA followed by Tukey’s multiple comparisons test. Significance was assumed at p < 0.05 and is indicated by asterisks: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0. 0001

Article Snippet: To identify genetic determinants of phage resistance, we performed whole-genome sequencing on resistant isolates and mapped the reads to the parental K. quasipneumoniae ATCC 700603 reference genome.

Techniques: Comparison, Staining

Journal: Brazilian Journal of Microbiology

Article Title: Molecular characterization and susceptibility of foodborne Escherichia coli to conventional antibiotics and natural extracts of Solanum palinacanthum and Siparuna guianensis

doi: 10.1007/s42770-026-01898-9

Figure Lengend Snippet: Percentage of multidrug-resistant E. coli strains present in food

Article Snippet: Quality control for the antimicrobial susceptibility tests was ensured by using the reference strain Escherichia coli ATCC 25,922, in accordance with the CLSI M100 35th edition (2025) guidelines [ ].

Techniques:

Journal: Brazilian Journal of Microbiology

Article Title: Molecular characterization and susceptibility of foodborne Escherichia coli to conventional antibiotics and natural extracts of Solanum palinacanthum and Siparuna guianensis

doi: 10.1007/s42770-026-01898-9

Figure Lengend Snippet: Percentage of E. coli strains resistant to antimicrobial groups present in food. ß-lac - ß-lactams; Qui - quinolones; Tetra - tetracyclines; Ami - Aminoglycosides; Amp - amphenicols; Sulf - sulfonamides; Lin - lincosamides; Pol - polymyxins; Mac – macrolides

Article Snippet: Quality control for the antimicrobial susceptibility tests was ensured by using the reference strain Escherichia coli ATCC 25,922, in accordance with the CLSI M100 35th edition (2025) guidelines [ ].

Techniques:

Journal: Brazilian Journal of Microbiology

Article Title: Molecular characterization and susceptibility of foodborne Escherichia coli to conventional antibiotics and natural extracts of Solanum palinacanthum and Siparuna guianensis

doi: 10.1007/s42770-026-01898-9

Figure Lengend Snippet: Distribution of phylogenetic groups of E. coli isolated from food

Article Snippet: Quality control for the antimicrobial susceptibility tests was ensured by using the reference strain Escherichia coli ATCC 25,922, in accordance with the CLSI M100 35th edition (2025) guidelines [ ].

Techniques: Isolation